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tandem tcf lef binding sites  (Addgene inc)


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    Structured Review

    Addgene inc tandem tcf lef binding sites
    Tandem Tcf Lef Binding Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tcf+lef+binding+sites/7TGP+(Plasmid+%2324305)/bio_rxiv__64898__2026__03__25__714063-210-25-29
    Average 93 stars, based on 50 article reviews
    tandem tcf lef binding sites - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia
    Article Snippet: .. The conditioned media was collected and added to HEK293T cells which were transiently transfected with a WNT reporter plasmid (Addgene #12456, M50, Super 8x TOPFlash) or WNT reporter control plasmid with mutated TCF/LEF binding sites (Addgene #12457, M51, Super 8x TOPFlash mutant) . ..

    Plasmid Preparation:

    Article Title: Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia
    Article Snippet: .. The conditioned media was collected and added to HEK293T cells which were transiently transfected with a WNT reporter plasmid (Addgene #12456, M50, Super 8x TOPFlash) or WNT reporter control plasmid with mutated TCF/LEF binding sites (Addgene #12457, M51, Super 8x TOPFlash mutant) . ..

    Control:

    Article Title: Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia
    Article Snippet: .. The conditioned media was collected and added to HEK293T cells which were transiently transfected with a WNT reporter plasmid (Addgene #12456, M50, Super 8x TOPFlash) or WNT reporter control plasmid with mutated TCF/LEF binding sites (Addgene #12457, M51, Super 8x TOPFlash mutant) . ..

    Article Title: A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis.
    Article Snippet: .. The TOPflash β-catenin reporter construct containing the Tcf/Lef binding sites and the FOPflash control reporter containing mutated Tcf/Lef binding sites were gifts from Randall Moon (Addgene plasmids #12456 and #12457). .. Plasmids driving the expression of mouse β-catenin deltaGSK (β-Cat GSK) and S33Y were gifts from Tannishtha Reya and Shinya Yamanaka, respectively (Addgene plasmids #14717 and #13371).

    Binding Assay:

    Article Title: Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia
    Article Snippet: .. The conditioned media was collected and added to HEK293T cells which were transiently transfected with a WNT reporter plasmid (Addgene #12456, M50, Super 8x TOPFlash) or WNT reporter control plasmid with mutated TCF/LEF binding sites (Addgene #12457, M51, Super 8x TOPFlash mutant) . ..

    Article Title: A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis.
    Article Snippet: .. The TOPflash β-catenin reporter construct containing the Tcf/Lef binding sites and the FOPflash control reporter containing mutated Tcf/Lef binding sites were gifts from Randall Moon (Addgene plasmids #12456 and #12457). .. Plasmids driving the expression of mouse β-catenin deltaGSK (β-Cat GSK) and S33Y were gifts from Tannishtha Reya and Shinya Yamanaka, respectively (Addgene plasmids #14717 and #13371).

    Article Title: Functional and Biological Characterization of the LGR5Δ5 Splice Variant in HEK293T Cells
    Article Snippet: .. The transcription factor TCF/LEF is activated by interaction with the coactivator β-catenin and binds specifically to the seven native TCF/LEF binding sites (TOPFlash M50, Addgene (#12456)) or less well to the six mutated TCF/LEF binding sites (FOPFlash M51, Addgene (#12457) (Addgene, Watertown, MA 02472, USA) as a background match). .. As an internal standard, renilla luciferase (from renilla reniformis, rLuc (pGL4.73 [hRluc/SV40] Promega, Mannheim, Germany)) with different substrate specificities was co-transfected under the control of a constitutive promoter and measured after firefly luciferase quantification.

    Article Title: Functional and Biological Characterization of the LGR5Δ5 Splice Variant in HEK293T Cells.
    Article Snippet: .. The transcription factor TCF/LEF is activated by interaction with the coactivator β-catenin and binds specifically to the seven native TCF/LEF binding sites (TOPFlash M50, Addgene (#12456)) or less well to the six mutated TCF/LEF binding sites (FOPFlash M51, Addgene (#12457) (Addgene, Watertown, MA 02472, USA) as a background match). .. As an internal standard, renilla luciferase (from renilla reniformis, rLuc (pGL4.73 [hRluc/SV40] Promega, Mannheim, Germany)) with different substrate specificities was co-transfected under the control of a constitutive promoter and measured after firefly luciferase quantification.

    Article Title: Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency
    Article Snippet: Next, puromycin-resistant cells were transduced with the neomycin-resistance sgRNA lentivirus and selected for 7 days with G418 (200 μg/ml, geneticin, ant-gn-1, InvivoGen). .. Establishment of dual-luciferase reporter cell lines For the dual-luciferase assay, a lentiviral EF-1α promoter-induced Renilla luciferase (pLX313- Renilla luciferase; 118016, Addgene, a gift from William Hahn & David Root), lentiviral Wnt reporter consisting of a tandem repeat of seven TCF/LEF binding sites (pXL010-Wnt dual (GFP-Fire) reporter; 40588, Addgene, a gift from Sean Palecek) and TEAD-YAP/TAZ reporter consisting of multimerized TEAD binding sites (HOP-flash; 83467, Addgene, a gift from Barry Gumbiner) were used. .. Establishment of dual-luciferase reporter cell lines For the dual-luciferase assay, a lentiviral EF-1α promoter-induced Renilla luciferase (pLX313- Renilla luciferase; 118016, Addgene, a gift from William Hahn & David Root), lentiviral Wnt reporter consisting of a tandem repeat of seven TCF/LEF binding sites (pXL010-Wnt dual (GFP-Fire) reporter; 40588, Addgene, a gift from Sean Palecek) and TEAD-YAP/TAZ reporter consisting of multimerized TEAD binding sites (HOP-flash; 83467, Addgene, a gift from Barry Gumbiner) were used.

    Article Title: Molecular differences of angiogenic versus vessel co-opting colorectal cancer liver metastases at single-cell resolution
    Article Snippet: .. TOP/FOP-Flash reporter plasmids containing intact or mutated Tcf/Lef binding sites (Addgene, 12,456; Addgene, 12,457) and Renilla plasmids (Promega, E2241) were co-transfected in H1299 cells using Lipofectamine3000 (Invitrogen, USA; 15,282,465). .. Transfected H1299 cells were treated with PDO supernatant (R-Spondin-reduced PDO tumor media conditioned by PDOs for two and seven days) for 16 h. The Dual Luciferase Reporter Assay System (Promega, E1910) was used to measure luciferase activities.

    Mutagenesis:

    Article Title: Widespread intronic polyadenylation inactivates tumor suppressor genes in leukemia
    Article Snippet: .. The conditioned media was collected and added to HEK293T cells which were transiently transfected with a WNT reporter plasmid (Addgene #12456, M50, Super 8x TOPFlash) or WNT reporter control plasmid with mutated TCF/LEF binding sites (Addgene #12457, M51, Super 8x TOPFlash mutant) . ..

    other:

    Article Title: Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency
    Article Snippet: The first lentiviral vector contained a Renilla luciferase reporter plasmid (pLX313-Renilla luciferase reporter; 118016, Addgene, a gift from William Hahn & David Root).

    Article Title: Recurrent WNT pathway alterations are frequent in relapsed small cell lung cancer.
    Article Snippet: Three micrograms of total RNA was used to make cDNA using the SuperScript II 1st Strand Synthesis Kit from Life Technologies (Carlsbad, CA, USA) with oligo dT primer according to the manufacturer’s instructions.

    Construct:

    Article Title: A Reciprocal Interaction between β-Catenin and Osterix in Cementogenesis.
    Article Snippet: .. The TOPflash β-catenin reporter construct containing the Tcf/Lef binding sites and the FOPflash control reporter containing mutated Tcf/Lef binding sites were gifts from Randall Moon (Addgene plasmids #12456 and #12457). .. Plasmids driving the expression of mouse β-catenin deltaGSK (β-Cat GSK) and S33Y were gifts from Tannishtha Reya and Shinya Yamanaka, respectively (Addgene plasmids #14717 and #13371).

    Luciferase:

    Article Title: Combined inhibition of Bcl-2 family members and YAP induces synthetic lethality in metastatic gastric cancer with RASA1 and NF2 deficiency
    Article Snippet: Next, puromycin-resistant cells were transduced with the neomycin-resistance sgRNA lentivirus and selected for 7 days with G418 (200 μg/ml, geneticin, ant-gn-1, InvivoGen). .. Establishment of dual-luciferase reporter cell lines For the dual-luciferase assay, a lentiviral EF-1α promoter-induced Renilla luciferase (pLX313- Renilla luciferase; 118016, Addgene, a gift from William Hahn & David Root), lentiviral Wnt reporter consisting of a tandem repeat of seven TCF/LEF binding sites (pXL010-Wnt dual (GFP-Fire) reporter; 40588, Addgene, a gift from Sean Palecek) and TEAD-YAP/TAZ reporter consisting of multimerized TEAD binding sites (HOP-flash; 83467, Addgene, a gift from Barry Gumbiner) were used. .. Establishment of dual-luciferase reporter cell lines For the dual-luciferase assay, a lentiviral EF-1α promoter-induced Renilla luciferase (pLX313- Renilla luciferase; 118016, Addgene, a gift from William Hahn & David Root), lentiviral Wnt reporter consisting of a tandem repeat of seven TCF/LEF binding sites (pXL010-Wnt dual (GFP-Fire) reporter; 40588, Addgene, a gift from Sean Palecek) and TEAD-YAP/TAZ reporter consisting of multimerized TEAD binding sites (HOP-flash; 83467, Addgene, a gift from Barry Gumbiner) were used.



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